Scientific Report of the Milan School of Medicine 2021; December 7 2021
Scientific evidence of anti COVID-19 mRNA and vectorial
vaccines genotoxicity inducing tumors and psycho-neuro-behavioral disorders.
DOI:
10.13140/RG.2.2.29151.18081
Giuseppe R.Brera[1]
Summary
. Micro-RNAs (miR) are non-coding RNA filaments
that control mRNA transcription. Micro-RNAs have been studied in cancer
pathogenesis, metastasization, cancer therapy, the structuring of the central
nervous system, diabetes, and heart disease.
Mir-134-138 regulate the development of dendritic spines needed for
synapses. Their silencing can lead to autistic spectrum disorders and mental
retardation and damage to brains in evolution such as childhood and
adolescence, producing learning problems and mood problems, and in adults for
alterations of receptors for neurotransmission.
It has been shown that N1-methyl pseudouridine binds to miR and induces
silencing processes, increasing cell methylome at the origin of cancer. The
production of mRNA vaccines replaces Uridine with N1-methyl-pseudouridine to
escape innate immunity and implement rapid translation.
N1-methyl-pseudouridine binding with
mi-RNA alters the epigenetic transcription of oncosuppressor that, with the
increase in cell methylation, could result in the induction of tumors and
relapses, natural immunity inhibition, and neuro-behavioral disorders
transmissible to progeny. Vectorial vaccines hybridize the host DNA with
adenoviruses and induce tumors at the experimental level. Clinical reports and
long-term epidemiological investigations are necessary to verify the impact of
mRNA vaccines on health.
The
SARS-COV 2 pandemic developed in China most likely for a probable laboratory
induction linked to the attempt to create a hybrid virus "SARS-COV-HIV"
for a vaccine or other purposes; the pandemic beginning determined a race to
the vaccine by "Big-pharma in opposition to the SARS-COV 1( 2002) and MERS
pandemics. (2009)" disappeared without mass vaccinations. Since 2002 until
2019, BIG-Pharma omitted to invest in anti-SARS-COV vaccines aware that the
speed of mutations of SARS-COV like HIV
would have made research unuseful. World
countries public health assessors, instead of determining a secondary
prevention strategy to protect o people with comorbidities at risk of lethality
(92%) based on a careful study of the literature on SARS-COV 1 for preventive
purposes, wholly omitted the person-centered indeterministic approach to
medicine inspired to Person-Centered Medicine, the medical science paradigm
revolution[2]
[3]
[4]
[5]
[6]
[7]
The omissions of the WHO and national governments, based on an illiterate
epistemological error leading to an approach to pandemics with a mechanistic,
linear model: "virus-infection-
disease-death risk and not virus-allostasis-natural immunity-vulnerability
risk-disease risk- death risk" oriented only to a mechanistic adaptive
immunity induced by experimental genetic vaccines not tested for long-term
adverse effects with insufficient and criticized trials.[8]
[9]
This illiteracy-based error in promoting global health has resulted in a
preventive strategy failure affecting human rights and the economy, leading to
5 million deaths and in Italy to about 150,000 (at the date). In Italy,
legislation induced vaccination with blackmail, such as the loss of work which
showed only for older than 39 a preventive efficacy in intensive care
admissions rate and lethality.[10]
Epidemiological
elaboration of data from the Italian "EPICENTRO
Istituto Superiore di Sanita'" ( October 9-November 10) shows in oldest
people > 80 after two doses of vaccines
< 6 months an increase of
lethality risk compared with unvaccinated (OR 1.59-IC q.1.2356 to 2.0587
P=0.0003 and a non-significant increase of lethality in the vaccinated range 12-39: OR 1.2-CI
0.1691-8-8229
In oldest people > 80, there is a higher mortality rate (OR
= 1.5949, p < .001) for the full vaccinated (two doses) within 6 months
compared to unvaccinated and a tendency in 12-39.
There is a tendency to increase risk of lethality in the vaccinated
compared to unvaccinated in the range 60-79 close to 0,5 significance: OR 1.23
( CI 0.5686 to 2.6962-P =0,5904) present in 60-79 people.
In > 80, the lethality risk of
vaccinated people is less than the full cycle of vaccination. (OR = 1.07 ,CI
0.5397 to 2.1330P = 0.5904. In other ages, first doses and full cycle of
vaccination appear to be a protective factor from lethality, < 6 months from
the first dose.
To date, the vaccines' failure to determine a durable immunity longer than
3-4 months for vectorial vaccines and six months for mRNA vaccines induced
public health assessors to induce a third dose boosting without any
consideration of adverse effects and possible alternative preventive measures.[11]
The
Italian population has been inundated with statements by the central health
government and regional governments driven media inducing vaccination or by
mediatic virologists also based on false public statements. It occurred with a
vaccination campaign for children, adolescents, and young people, not at risk
of COVID-19, that if rarely infected, they are asymptomatic and with a ratio of
cases/fatality to zero or almost. Roberto Speranza, the Italian Health Minister,
declared "The full agreement of all scientists" about the need for
vaccination in all ages." Franco Locatelli, the director of the Anti
SARS-COV Italian Technical, stated on August 20 the inexistence of adverse
effects for adolescents to induce parents' authorization after the news of
healthy adolescents' deaths after vaccination from Italy and USA and signalization
of adverse effects by USA CDC, also in contrast with health policy of countries
like the UK.
Lethality
from COVID-19 concerns 92,8% of people with comorbidities [12]
characterized by atherosclerosis which leads to an auto-immune phenotype and
immune anergy after the 7th day of the disease, the actual cause of
lethality [13] confirming the theories
of the relativity of the infection to cholesterol concentration in lipid rafts
and the caveolar lipid rafts number and the probability of severe clinical syndrome
relative to the LDL/HDL ratio and phospholipase concentration in the cell
membrane, one factor altering the immune signal transduction. [14]
Recently,
Maurizio Federico, with a significant review[15]
in a very profound and straightforward way, highlighted that the mRNA vaccines
have hard limits in immunogenicity. Vaccines have limited usefulness in time
and are restricted to RBD of viral S-proteins of the original viral strain,
losing effectiveness with variants because of the "Original antigenic sin.
Vaccines do not stop contagions because they do not produce neutralizing
antibodies (IGA) in mucous membranes of the
pharynx and upper respiratory ways, so laws that lead to the obligation to vaccination
certificates appear without any scientific reason. They do not induce resident memory B-cells in
lungs, not preventing the first cause of lethality, but only IGG in the
bloodstream. Moreover, they select
variants whose viral allostasis completely escape any previously vaccine-induced
immunity. Moreover, restrictions hamper the asymptomatics-and healed-induced
herd immunity, while there is the possibility to treat the infection early with
efficacy,[16] identifying people at
risk with the probability theory of the COVID-19 severity. [17]
In the
light of the only IGG stimulation and
the lack of activation of resident memory B-cells in the lung, the antiviral
effectiveness of mRNA vaccines is a conundrum.
Viruses lead to asymptomatic infections,
depending on innate and adaptive immunity, as happens for most people every
week with different species of virus and that for SARS_COV 2 is due to the
immunization from other non-dangerous coronaviruses, such as
corona-adenoviruses which target at least 50% of people who reach a partial
immunity also to SARS-COV 2, because of common antigens. On the other hand, infections
depend on the protective factors that stimulate natural immunity, eliminating
the virus before it reaches the epithelial and endothelial cells. Infection is only possible on the condition of
degeneration of the cell membranes due to cholesterol and LDL, inducing the
formation of lipid rafts whose caveolae are the obligatory gates to the
infection.[18] The oldest people with
atherosclerosis–based comorbidities are more at risk of lethality because of
immune anergy. Conversely, infections are rare and almost asymptomatic, with a
low infectivity index in children and young people with healthy cell membranes.
It has been computed that corona-adenoviruses immunize at least 50% of people.
Antibodies against these neutralize vectorial vaccines adenoviruses vectors. Vaccinations with viral vectors are
dangerous for children and adolescents closer to infections for the intensity
of immunity reactions leading to the risk of disease from immunocomplexes and
an increased thrombophilia.[19]
[20] In Italy and adolescents died of thrombosis
after the boosting dose of AstraZeneca.
The
vaccines authorized by the Italian AIFA are genetic because they use genetic
mechanisms to induce an artificial immunity against a particular
"Spike-protein" defined by a messenger RNA code of the Huwan strain
that allows the activation of adaptive cell immunity through activated
lymphocytes T and B that with their proliferation assure a minimal immunity
over time, not inhibiting the contagion. [21]
The mRNA
vaccines are constructed with a sequence of bases homologous to RBD of the
viral spike protein. The code is then transcribed by the RNA transfert, which
allows the viral protein synthesis in the cytoplasm and in the organelles
(endoplasmic reticulum and Golgi apparatus) to form the new virus. The mRNA
vaccine is conveyed by lipid nano-particles that favor its entry into the cell
membrane. The infected caveolae of the lipid rafts alter the transduction of
immunity signals inhibiting proliferation and activation of lymphocytes T and B,
but this is undoubtedly related to the alteration of the cell membrane and the
flooding of macrophages with cholesterol and PH. Immunosenescence and the previous
immune-atherosclerosis phenotype inhibit adaptive immunity and memory-t and b
cells production. [22]
The virus dissemination is relative to a previous immunosuppressive phenotype
and "inflammaging" induced by atherosclerosis inducing a lethality
risk for the oldest people with comorbidities associated with atherosclerosis,
like diabetes, obesity, hypertension, cardiovascular diseases. It explains the
higher rate of mortality of the immunosenescent oldest people. The SARS-COV 2
Immunity hijacking appears mediated by MTTL3, which blocks the RIG-1 receptors
recognition.[23]
The mRNA
vaccines induce a high IFN gamma reaction and stimulate CD4 TH1 cells in local
lymph nodes, but neither induce the antiviral CD8+ mediated immunity[24]
because they do not stimulate synthesis nor interact with the lung B-cell
memory-resident cells, not activating these. The immunity induction of mRNA
vaccines is a "conundrum." [25]
The
heterologous "mRNA" inoculated with the vaccine "infects" all the immune, epithelial, endothelial cells,
neurons in every anatomical structure, from the brain to the heart, the
endocrine organs, and the toe.
The problem that
viro-immunologists[26]
had to face was to prevent the inoculated heterologous m-RNA from being recognized by Toll-like receptors (TLR). [27]
For this purpose, in m-RNA, they replaced
a base: Uridine with n1-methyl pseudouridine,
which escapes immune control of the inoculated host and increases
translation speed. In
2019 J. Lockhart, J Canfield J, Mong EF et al.[28] demonstrated that the replacement of the
Uridine of the Spike Protein mRNA with the n1methyl-pseudouridine that is necessary
for the mRNA production alters the silencing of micro-RNA switches leading to a
decrease in the activity of these molecular switches, called "the dark
matter" of the cell (about 50% of RNA) and thus altering the processes of
silencing. What
happens if the repressor of an oncogenesis inhibitor is not silenced?
N1-methyl-pseudouridine
binds to miR, altering their action to silencing miRNAs, possibly harming the
organism's life. Micro-RNAs are filaments of non-coding RNA that, thanks to
the "Argonaut" proteins after joining the "RISC (RNA-induced
silencing complex, RNA-induced silencing complex), interact internally with the
target RNA, preventing transcription by preventing the synthesis of the protein
with the specific mRNA silencing. After
their discovery in 1993 by Victor
Ambros, Rosalind Lee, and Rhonda Feinbaum, the role of particular microRNAs was
studied in pathogenesis and cancer therapy,
in the structuring of the central nervous system, in diabetes, in heart disease. For example, if mi-RNA 205 is inhibited in
the pathogenesis of breast cancer, carcinogenicity and metastasisation are
encouraged. The same occurs for mi-RNA 21 for liver cancer. In 2009 G. Schratt,
with a great contribution, illustrated some fundamental actions of miR in
neurons. Mir-134-138 regulate the development of dendritic spines needed for
synapses.[29] Their alteration can lead to autistic spectrum disorders and
mental retardation and in brains in development as in childhood and adolescence,
to learning and mood problems, as well as in adults to neuro-transmission
receptor alterations such as CAMKII and CREB.
Regulation of innate immunity involves mi-RNA 155-146 -132 as illustrated by J. Raisch,
A.Darfeuille-Michaud, HT. Nguyen in their elegant review of 2013. Mir-155 regulates the suppression of the
cytokine signaller (SOCS)-1, which negatively regulates the capacity of
the "Antigen Presenting Cells " APC to present antigen and activate
lymphocytes.[30] [31] Cells with the
lack of mir-155-show a defective presentation of antigen and therefore cannot
activate T cells to promote the TH1-induced inflammation [32]:
this could be the epigenetic pathogenesis of anergia and immunosenescence.
Another study has shown that the elimination of mir-155 expression
significantly increases the expression of the pro-inflammatory IL1. These observations depict how mRNA vaccines
could induce paradoxical inhibition of innate immunity, increasing people's
vulnerability to infection and cancer. In atherosclerotic M2 immune phenotype,
present in comorbidities at risk of COVID-19 severity,[33]
it could induce anergy when there are other infections with SARS-COV 2 variants
escaping previous and waning adaptive immunity in short-time, exposing oldest immunosenescent
people to a clinical syndrome severity up to lethality. Moreover, the induced
methylation by mRNA vaccines, resulting in an MTTL3 cellular increase, supports
the viral hijacking of immunity by SARS-COV 2 variants, and reducing natural
immunity. It means that more mRNA vaccines are inoculated, more natural
immunity is reduced, making easier variants’ immunity hijacking.
Numerous studies document how dysregulation of mi- RNA is
associated with cancer development and metastasis processes, as documented by
the splendid review of G. Sotiropoulou, G. Pamplakis, E.Lianidou Pampalakis,
Lianidou, Z. Mourelatos. Cancer
pathogenesis is associated with several bio-molecular processes such as genomic
alterations, transcription of oncogenic factors, and inhibition of repressors
transcription, such as P53 and hypoxia.
Epigenetic
changes are regulated by micro-RNAs which are the arbiters of cell health as
on/of molecular switches of mRNAs.
Viral
mRNAs such as mRNA vaccines act by altering microRNAs. The mRNA vaccines act
like a virus at the epigenetic level.
Recently E Karimi, H. Azari Yari, M, Tahmasebi, et al.
identified 39 mi-RNA derived by Sars-COV
2 inducing a viral allostasis inhibiting the innate immunity, altering Vit. D
and the lung cells metabolism through the transcription alteration.[34]
The
silencing of miR-223 appears to be caused by an epitranscryptomic alteration of
pre-micro-RNA, which produces an oncogenic factor that binds to its site,
producing its "switching off." This is associated with leukemia
pathogenesis. An epidemiological study on the incidence of post-vaccine
leukemia and other cancers is needed. The direct induction of miR dysregulation
produced by mRNA vaccines can have dramatic consequences for millions of young
people and children by inducing the pathogenesis of tumors or relapses and
diseases of the central nervous system. What will be the effect of mRNA
vaccines on the brain of the ruling class since their action determines
alterations of the miRNA that control neuronal nuclei biological substrate of
the cognitive and subcortical sphere.
What will happen with the impairment of the activity of the cerebral
cortex and the subcortical nuclei?
There is a pandemic of illiterate criminality
in people who want to induce the vaccination of adolescents and children who
are not at risk of COVID-19. If rarely infected, children are asymptomatic[35] thanks to their solid
innate immunity and rapidly reduce the viral load in the oral and nasal mucous
membranes. Children and adolescents and
asymptomatic people could act as "living vaccines" contributing to
the" herd immunity" as often occurs with other viruses. It has been
well highlighted that asymptomatic people relatively contribute to the virus
diffusion. Vaccination exposes children and adolescents to epigenetic and
genetic damages whose impact must be studied with epidemiological
investigations but is sure.
Two meta-analyses show the reduced infectivity of
asymptomatic people (AIC). Transmission rates of AIC ranged from 0–2.2%
compared to 0.8–15.4% for symptomatic (SIC)[36]and in the household
from 0–4.9% compared to 18.0% of SIC. [37]
.
The infectivity reduction
of asymptomatic people is probably due to the presence of neutralizing IGA in
mucous membranes, absent in vaccinated people.
A study that monitored
455 contacts exposed to the asymptomatic COVID-19 virus carrier showed that
nobody was infected.[38]
The reduced viral load
transmission by the upper respiratory and its disappearance in a shorter time
can explain this evidence and reverses the common belief that asymptomatic people
induce the pandemic. Conversely, a reduced transmissible viral load to healthy
people could activate the people's innate immunity and the progressive loss of
virulence by activating the tissue-resident memory T cells that block the virus
diffusion in the organism.
The right strategy to
accelerate herd immunity is a health education campaign to educate to assume
immunogenic molecules (e.g., beta-glucans in bakers yeast) and inhale vapors of
powerful common natural antiviral substances at the first signals of infection
of upper respiratory ways.[39]
The SARS-COV 1(
2002-2003) and MERS (2009-2010) disappeared without vaccines.
What
will happen in millions of adolescents and young people not at risk of COVID-19
that the Italian health management and other countries led to vaccination with a
legalized blackmail, because of the alteration of the regulation of mir-223,
whose alteration is linked to the pathogenesis of leukemia? In the USA, the FDA
recently approved mRNA vaccines in children based on a small Pfizer trial,
which used children as experimental animals and that did not monitor the
adverse effects at the epigenetic level and their manifestation after a long
time.
The other process
induced by mRNA vaccines is the cell's methylation induced by
n1-methylpseudouridine. The n1-methylpseudouridine (ɸ)
stabilizes the RNA. It is naturally present more in the RNAt, with implicit
natural finalism to favor the coding probably. In synthesizing the mRNA
vaccines, the Uridine has been replaced with ɸ
to increase the translation speed and evade the natural immunity. However,
introducing ɸ in each cell produces
cellular stress that could be equivalent to "heat stress" that produces an 'increase of cell methylome
by methylating all the bases through the synthesis induction of METTL3
(methyltransferase like 3) with the finalism to ensure an allostasis for
survival. The METTL3 binds to microRNA, causing the down-regulation of some and
up-regulation of others. The action of miR like that of antisense RNA could
produce the silencing of the P15 gene, which encodes a dependent cycline kinase
involved as a repressor of malignant leukemic degeneration. W Yu, D Gius, P. Onyango, et al. in 2008 saw an
inverse relationship between P15 and leukemia, highlighting the risk of its
repression. Antisense RNA also interacts directly or indirectly with
DNA-methyltransferase leading to DNA methylation and its consequences in the
repression of gene transcription and the recruitment of "histone-modifying
enzymes" by modifying chromatin.
This evidence means
that in opposition to an illiterate bio-medical culture, mRNA vaccines also
induce genetic alteration that, in addition to induction or suppression of miR,
lead to DNA methylation and modification of chromatin.
Moreover, the increase
in cell methylome could be caused by the lack of repression of the MTTL3
synthesis by silencing its mRNA induced by a specific micro-RNA. The MTTL 3
increase induces the general methylation of nucleotidic bases with catastrophic
effects, a sort of earthquake in the organism's life, with dramatic effects on
the pathogenesis of tumors.
In the tissues of
patients with "Non-small cell lung
cancer (NSCLC) (small cell lung cancer) "N6-methyladenosine (m6A)
methyltransferase-like 3 (METTL3)" regulates microRNA-1246 (mir-1246) which is a well-documented tumor's
progression and metastases. More METTL3 and mir-1246 were found in these
tissues in inverse ratio to PEG-3 ( Paternally expressed gene-3).[40]
The alteration of micro-RNA is, therefore, very dangerous.
This confirms the aversion of Luc Montagnier, Nobel Prize, against the use of
genetic vaccines before knowing in depth their effects with long-term epidemiological
investigations.
Several deaths and
adverse effects on mRNA vaccines have already been reported worldwide, [41]
such as the high incidence of pericarditis and myocarditis in young people,
which in some countries like the UK, has blocked any other vaccination in
adolescents and young people, not at risk of COVID-19. In Italy, a teenager
died a few hours after the second vaccination by m-RNA, another from the
vectorial Astra-Zeneca. In the USA, the CDC
reported the deaths of 14 teenagers. The actual extent of these events
is entirely unknown because public health, organizations such as AIFA, the Ministry of Health, the Italian
Higher Institute of Health, to our knowledge, have not organized epidemiologic
research to study the adverse effects of these vaccines over time.
Undoubtedly the fear of
COVID-19, induced by a virus quickly and destroyable and
neutralizable with natural antiviral remedies also immunogenic and drugs
already in use, [42] that prevents contact
with epithelial and endothelial cells is derived from the lack of public health
orientation towards “Person Centered Prevention”[43]
, and to failure in primary and secondary prevention. This omission is due to
ignorance and the non-adoption in public health of the multi-factorial,
multi-dimensional paradigm of "Person-centered medicine," the
paradigm change of medical science, that could have saved only in Italy 150.000
and in the world millions of people. As Luc Montagnier claims, we need analysis
over a long time to control the existence of adverse effects, even fatal at a
short time. The only answer to the pandemic beginning and permanence has been
the adoption of "genetic" vaccines, which have been inadequately
tested and limited in time, with the scientific evidence of their danger. The
adverse effects of these vaccines and the induction of variants endanger the
health of millions of people and must be prevented or blocked in time to avoid
a global health disaster, primarily protecting children and adolescents whose
health is to date menaced by mRNA vaccines.
The mRNA vaccines'
dangers, as highlighted above, are shared by the viral vector vaccines. These induce a modification of human
DNA because of the recombination of the animal or human DNA vector adenovirus with the
host's DNA. This hybridization could lead to auto-immune reactions and, at the
experimental level, to an impressive induction of tumors. [44]
Scientific evidence of the anti SARS_COV 2 vaccines- induced
genetic damages must be studied with clinical and epidemiological
investigations. However, before it is necessary a total change of public health
administration resulting in the withdrawal of the authorizations to mRNA
vaccines distribution and the constitution of a metabolic and immune shield for
the population by adopting the "Antiviral allostasis, and
immuno-stimulation"[45] strategy launched in
Italy by the National Health Committee and in the world by the World Health
Committee.
Unfortunately,
the prudence towards vaccines that led to their rejection only by a minority of
the population and health care workers has a sound scientific basis. The
dramatic suspension of health care workers who refused vaccination by a
surprising (in the negative sense) physician's council and discrimination of
workers paradoxically supported by unions - but unions should not defend workers?
-that do not want to vaccinate is without any scientific basis.
What can also happen to the brain, mind, and
behavior of the national leadership, institutional public or business, teachers,
and in any context with the genes of the adenovirus of chimpanzees integrated
into the genome of cortical neurons or sub-cortical brain (Astra-Zeneca
vaccine) that, if in age, communicate to the progeny? Will the COVID-19 contribute to human evolution?
Clinical reports and
long-term epidemiological investigations are necessary to verify the impact of
mRNA vaccines on health.
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